![]() ![]() The activities of treated IgY and IgG were tested by enzyme linked immunosorbent assay (ELISA). Commercial antitetanus IgG was used as control. 120).The purified IgY was then treated at different levels of temperatures and pH as well as proteolytic enzymes. IgY was purified by ammonium sulfat precipitation and gel filtration chromatography (Sephadex G. ![]() Serum samples were collected 4 weeks following the last immunization. Antitetanus IgY was produced by immunization of chickens with tetanus toxoid, three times weekly at gradual doses of 100, 200, and 300 Lf, respectively. ![]() A study was carried out to find out an alternative method of producing antitetanus antibody (IgY) in chicken and to evaluate its activity at different levels of pH, temperatures and proteolytic enzymes.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |